Journal: PLoS ONE

Article Title: Pre-Clinical Assessment of Novel Multivalent MSP3 Malaria Vaccine Constructs

doi: 10.1371/journal.pone.0028165

Figure Lengend Snippet: The antigens in the PP constructs are colour-coded as follow; MSP3-1 (AA 184 to 251) in red, MSP3-2 (AA 161 to 257) in light green, MSP3-3 (AA 228 to 307) in orange, MSP3-4 (AA 508 to 579) in dark brown, MSP3-7 (AA 214 to 285) in dark blue, MSP3-8 (AA 537 to 624) in dark green. The construction PP8 is constituted by the assembly of the amino acid sequences 161–257 to 293–371 of MSP3-2. The amino acid sequences in the PPs correspond to the sequences of the clone 3D7 of P. falciparum . PP3 and PP4 contain p27 and LSA3 linkers respectively and are quoted in black on the diagram. The histidine tag appears in gray at the C-terminal extremity. The poly-bcd polyprotein construct designed previously which incorporates all the “b–d” regions of the six MSP3-CT antigens is also shown, for comparison. The size and theoretical molecular weight of the polyproteins produced in E. coli are indicated.

Article Snippet: Nucleic acid sequences coding for the MSP3 polyproteins (PPs) were chemically synthesized (GenScript).

Techniques: Construct, Comparison, Molecular Weight, Produced

Journal: PLoS ONE

Article Title: Pre-Clinical Assessment of Novel Multivalent MSP3 Malaria Vaccine Constructs

doi: 10.1371/journal.pone.0028165

Figure Lengend Snippet: Kinetic of immunogenicity of PPs against each of the MSP3-CT antigen was investigated in mice of three distinct genotypes. Total IgG response was measured every two weeks after each immunization and expressed as mean OD 450 value. The arrows on the x-axis indicate the timing of the subcutaneous injections of 20 µg of recombinant PPs in Montanide ISA720 adjuvant. In the particular case of MSP3-1 values are only indicative as they are higher than OD = 5, i.e. than the linear range of the reader.

Article Snippet: Nucleic acid sequences coding for the MSP3 polyproteins (PPs) were chemically synthesized (GenScript).

Techniques: Immunopeptidomics, Recombinant, Adjuvant

Journal: PLoS ONE

Article Title: Pre-Clinical Assessment of Novel Multivalent MSP3 Malaria Vaccine Constructs

doi: 10.1371/journal.pone.0028165

Figure Lengend Snippet: Total IgG titers were measured two weeks after the third immunization of C5BL/6 (A), BALB/c (B) and Swiss (C) with MSP3 polyprotein constructs. IgG titers for LSA1 or adjuvant immunized mice, not represented here, against MSP3 family antigens were insignificant. End point for IgG titer determination was a mean OD 450 = 0.2.

Article Snippet: Nucleic acid sequences coding for the MSP3 polyproteins (PPs) were chemically synthesized (GenScript).

Techniques: Construct, Adjuvant

Journal: PLoS ONE

Article Title: Pre-Clinical Assessment of Novel Multivalent MSP3 Malaria Vaccine Constructs

doi: 10.1371/journal.pone.0028165

Figure Lengend Snippet: Cytophilicity ratio (CR) of IgG response against MSP3-CT antigens was assessed in C57BL/6 (A), BALB/c (B) and Swiss (C) mice, two weeks after the third immunization with PPs. CR was measured as following (IgG2a+IgG2b+IgG2c)/(IgG3+IgG1+IgM). Results were expressed as the geometric mean of the OD 450 obtained for the single dilution of sera at 1∶1000.

Article Snippet: Nucleic acid sequences coding for the MSP3 polyproteins (PPs) were chemically synthesized (GenScript).

Techniques:

Journal: PLoS ONE

Article Title: Pre-Clinical Assessment of Novel Multivalent MSP3 Malaria Vaccine Constructs

doi: 10.1371/journal.pone.0028165

Figure Lengend Snippet: Western blotting of human erythrocytes infected by Plasmodium falciparum (3D7 clone) and probed with pooled immune sera from groups of mice two weeks (A) or six months (B) after the sixth immunization with polyproteins or with adjuvant alone (mock immunized).

Article Snippet: Nucleic acid sequences coding for the MSP3 polyproteins (PPs) were chemically synthesized (GenScript).

Techniques: Western Blot, Infection, Adjuvant

Journal: PLoS ONE

Article Title: Pre-Clinical Assessment of Novel Multivalent MSP3 Malaria Vaccine Constructs

doi: 10.1371/journal.pone.0028165

Figure Lengend Snippet: ADCI was performed with human MN and parasites cultured for 4 days with immune sera from C57BL/6 (A), BALB/c (B) and Swiss (C) mice harvested two weeks after the fifth immunization with the MSP3-PPs. Sera from mice immunized with an irrelevant P. falciparum antigen (LSA1) or with the adjuvant alone, were used as negative controls. A pool of immune sera from individuals living in endemic areas (PIAG) was used as positive control. The PIAG was used at a dilution of 10%, the mice immune sera were used at a 5-fold serial dilution of 2.5%, 0.5% and 0.1%. Results are expressed as adjusted SGI values compared to the PIAG value that was redressed to 100% of SGI effect.

Article Snippet: Nucleic acid sequences coding for the MSP3 polyproteins (PPs) were chemically synthesized (GenScript).

Techniques: Cell Culture, Adjuvant, Positive Control, Serial Dilution